Anti-Diabetic Potential of Gymnema Sylvestre: In Vitro and in Silico Analysis

Gymnema sylvestre (GS) is a powerful antidiabetic plant that has been utilized in ayurvedic, folk and homeopathic medicine for centuries. In this research, we evaluated the potential of methanolic leaf extract sylvestre. Fractionation was carried out using column chromatography total twenty-eight (28) sub-fractions were obtained which further screened pooled into three (3) fractions (A, B C) by thin layer based on their retention factor (Rf) values. The subjected to vitro α-amylase α-glucosidase inhibition activity. Some compounds identified from LC-MS silico analysis between all ligands receptors with aid docking software. Ligands imported visual screening PyRx software while Biovia Discovery Studio Visualizer used protein preparation. Analysis properties drug likeliness done via SwissADME online server according Lipinski’s Rule Five. Final through AutoDockVina client 2020. Fraction C showed best IC50 0.84µg/ml inhibitory activity when compared fraction A B, 2.00µg/ml 1.58µg/ml (α-glucosidase), produced among 16.78µg/ml, 23.17µg/ml 28.22µg/ml. Molecular Orcinol (-5.5 kcal/mol) strong binding interaction α-amylase, followed 3-hydroxy-3'-methoxyflavone Curcumin (-7.1 kcal/mol -7.6 respectively) acarbose (-8.0 Glyinflanin (-8.4 interactions. affinity Orcinol, 3-hydroxy-3'-methoxyflavone, (-5.7 kcal/mol, -8.0 -9.1 lower (-9.7 α-glucosidase. Thus, found have potentials displaying most effective development.